Otto Zuckerkandl was born in Raab, Hungary, as a younger brother of the renowned anatomist Emil Zuckerkandl. From 1912 he was assistant professor of the surgical department at the Jewish Community Hospital in Vienna. He was the most important assistant of professor Leopold Dittel, a surgical urologist.
Zuckerkandl worked primarily on and wrote about hypertrophy of the prostate, about the muscular system of the bladder, the histology of zystitis, and about tumor in the bladder.
He is the author, with Julius Tandler, of Handbuch der Urologie, (1903-1905). He was coauthor on a number of textbooks on urology and surgery, among them Handbuch der Therapie der Tukerkulose.